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It's the penultimate episode of Holby City next week, and there won't be a dry eye in the house as Jac undergoes life-threatening surgery ? but not before making a few final demands.

Elsewhere, Josh is confronted over his relationship with Claudia and Sacha has a huge realisation.

Here's a full collection of the eight biggest moments to watch out for:

1. Max has doubts about Jac's surgery

Elliot's plan to operate on Jac's tumour is thwarted when her latest scan results come through. With the situation now far more complicated than any of them could have anticipated, Max urges Elliot to put a stop to the surgery immediately.

Elliot is torn over what to do but Jac is resolute in her decision and urges Eliot to push on regardless.

Despite her uncertainties, Max reluctantly agrees and arranges for the operation to go ahead that day ? but insists that Jac must let them know if she suffers any further symptoms between now and then.

2. Jac's health takes a turn for the worse

Jac begins to reflect on her past as she prepares for the life-changing surgery that will determine her future. However, she is left panic-stricken when she suffers a frightening episode while alone, but manages to call Elliot for help just in time.

Elliot warns Jac that this has the ability to impact her operation and that they need to inform Max ? as previously agreed ? but she begs him not to.

3. Jac has an impossible demand for Elliot

As Jac prepares to go into theatre, she has an impossible demand for her surgeon Elliot.

Jac tells Elliot that she isn't prepared to keep on living with the debilitating symptoms of her illness and makes him promise to let her die on the table if he isn't able to remove the tumour in its entirety.

Elliot is left thrown by Jac's request, but what will happen when the operation gets underway?

4. Sacha makes a discovery

It's a tough week for Sacha, too, as he tries and fails to address his concerns over Jac's surgery.

After struggling to get through to anyone ? including Jac herself ? Sacha finds himself armed with a request, which changes everything.

Jac asks Sacha to fetch her locket, a keepsake for her late sister Jasmine, but makes an alarming discovery while at her flat.

Realising that Jac has accepted her fate, Sacha does his best to stop the surgery ? which is already underway ? but will Jac live or die? Trust us, you'll need the tissues for this one either way.

5. Eli and Amelia have some news

As Holby begins the process of wrapping up its many loose ends, there is a happy update for Amelia and Eli next week.

The couple are overjoyed to discover they are expecting again, and share their happy news with an emotional Jac.

6. Josh is questioned over his past

Josh seems to be in recovery from his bulimia relapse, until an unwanted visitor dredges up old ghosts.

Josh is shocked to get a visit from Claudia's grieving mum, who, inexplicably, believes he played a part in her death.

As Josh reels from the accusations over his relationship with Claudia, his day gets even worse when Dom confronts him over what's been going on as well.

7. Dom demands answers from Josh

Having found Claudia's diaries, a furious Dom accuses Josh of cheating on Ange.

Backed into a corner, Josh explains to Dom that he met Claudia in therapy for bulimia, and insists there was nothing more going on between them.

Josh later confides in Ange over the accusations, but how will she react?

8. Hanssen reconsiders his future

Returning Reverend Lexy receives good news about a new kidney.

She enjoys catching up on the lives of her old friends, but will her wise words provoke Hanssen to wonder what he really wants from life?

Holby City airs these scenes on Tuesday, March 22 on BBC One.